Work Package 3: Enzyme improvement
The aim of Work Package 3 “Enzyme improvement” is to develop enzymes with improved properties for industrial applications. Novel as well as selected FAEs and GEs available within the consortium will be improved for their thermotolerance, solvent-resistance and substrate specificity by directed evolution and site-directed mutagenesis.
In addition to standard characterisation on substrate specificity, the ability of the enzymes to perform the selected model biosynthetic reactions will be tested. The aim is to produce 50 novel fungal and 500 bacterial esterases, 25 site-directed and 20 directed evolved mutants.